Detailed Protocol

Every dataset published on the SPARC Portal MUST have a published protocol associated with it. One way to do this is to publish a protocol manuscript, another way is to publish your protocol using Protocols.io. For more details about this process visit Create Your Protocol.

Protocol Inclusion Requirements

Every protocol that will be accepted by the SPARC Curation Team must include the following:

  • Title- must be specific to the dataset
  • Abstract- a brief description putting methods in context
  • Experimental design - include the number of subjects, groups, treatments, measurements, and inclusion/exclusion criteria
  • Species used in the study - provide vendor name, catalog numbers, and Research Resource Identifiers (RRIDs). The species given in the protocol must match the species described in the associated dataset
  • Reagents, equipment, and tools used in the study – provide vendor names, catalog numbers, RRID, and links to supplier websites
  • Techniques and methods used in the study - must match the associated dataset

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RRIDs are ID numbers assigned to help researchers cite key resources (antibodies, model organisms, software projects, etc.) in the biomedical literature to improve the transparency of research methods. Learn how to search or request RRIDs from SciCrunch Resources

Digital Object Identifier

Protocols.io will provide you with a DOI when you officially publish the protocol. But you won’t publish at first – you’ll create a temporary link that the SPARC Team can use to review. Then, when given confirmation from us, you’ll publish to get the DOI.

Once received, the DOI should be provided to the SPARC Curation team immediately via email so the “dataset_description” metadata file and the Pennsieve website can be updated before the dataset publication.